Effect of temperature on the separation of some Δ9‐cis‐trans isomers of methyl esters of fatty acids by open tubular gas chromatography

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Abstract
The influence of temperature on the gas chromatographic separation ofcis‐trans isomers of the methyl esters of some monounsaturated fatty acids was studied on capillary columns coated with Apiezon L, BDS and DEGS. As far as methyl oleate and methyl elaidate are concerned, the separation is better at lower temperatures on Apiezon L (180–210 C) and at higher temperatures on polyester phases (BDS, DEGS; 150–180 C). The influence of temperature on the separation ofcis‐trans isomers on the three stationary phases under study is explained by the higher values of δECL/δt forcis isomers. The variation of the equivalent carbon chain length with temperature can be used for the identification ofcis‐trans isomers in natural mixtures.