Haemocyanin

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Abstract
Dialyzed serum was employed, changes in p H being effected by addition of KOH. Between p H 7.8 and 9.65 the O dissociation curve of Homarus hemocyanin is modified in 2 directions by pH change. Increase in alkalinity causes the initial portion to become steeper while the upper portion tends to approach saturation more and more asymptotically. At p H 9.65 the inflexion of the curve disappears, while the curve itself approximates a hyperbola, which would be expected if combination with O followed the law of mass action as formulated for homogeneous solutions. The inflexion at a lower pH is apparently connected with the fact that the pigment is present as a mixture of salt (with alkali) and uncombined substance. The isoelectric point of hemo-cyanin from Homarus is about p H 4.8 and that from Limulus about 6.3. Limulus hemocyanin differs from that of Cancer, Homarus, Maia, and Palinurus. Pigments from the latter 4 are also probably different.

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