cDNA-AFLP Display for the Isolation of Peronospora parasitica Genes Expressed During Infection in Arabidopsis thaliana
Open Access
- 1 August 2000
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 13 (8) , 895-898
- https://doi.org/10.1094/mpmi.2000.13.8.895
Abstract
To identify genes from the obligatory biotrophic oomycete Peronospora parasitica that are expressed during infection in Arabidopsis thaliana we employed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fragments from infected and non-infected leaves were separated in parallel by gel electrophoresis and displayed by autoradiography. Most differential gene fragments were derived from P. parasitica.Keywords
This publication has 7 references indexed in Scilit:
- The TIGR Gene Indices: reconstruction and representation of expressed gene sequencesNucleic Acids Research, 2000
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Characterization of eds1, a mutation in Arabidopsis suppressing resistance to Peronospora parasitica specified by several different RPP genes.Plant Cell, 1996
- Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.Proceedings of the National Academy of Sciences, 1996
- Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber developmentThe Plant Journal, 1996
- Recent Advances in the Genetics of Oomycete Plant PathogensMolecular Plant-Microbe Interactions®, 1996
- AFLP: a new technique for DNA fingerprintingNucleic Acids Research, 1995