Effect of Dilution on Fertilizing Capacity of Rabbit Spermatozoa

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Abstract
Twenty doe rabbits were superovulated by the method of Pincus and inseminated with a known number of sperm in 1, 0.4, or 0.1 ml. of saline. The does were killed 38-42 hrs. later. The ova were flushed out and the number of cleaved ova counted. The results show that 17-42%, 0-28%, and 0-6% cleavage of the ova occurred when a similar number of sperm (30,000-44,000) was suspended in 0.1, 0.4, and 1 ml. of saline, respectively. The mean % of cleavage (2.75) with the 1-ml. insemination was significantly different from the mean % (27) with the 0.1-ml. insemination. A maximum of 19% cleavage when the number of sperm was doubled (80,000) but suspended in 1 ml. of saline. The advantage of a small vol. of concentrated sperm is shown. In the diagnosis of [male] infertility, the vol. of semen in relation to number of sperm must be considered. In artificial insemination, a small vol. of semen with a high sperm concn. should be instilled.

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