Rapid miniaturized chromatography for Tc-99m IDA agents: Comparison with gel chromatography

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Abstract
Miniaturized chromatographic systems for determining free pertechnetate and hydrolyzed reduced 99mTc levels in comericial 99mTc-labeled iminodiacetate (IDA) hepatobiliary radio-pharmaceuticals were evaluated and the results compared with gel chromatography column scanning (GCS). Commercial IDA agents were evaluated including 2,6-dimethyl IDA, p-isopropyl IDA, p-butyl IDA, and 2,6-diisopropyl IDA. Of all the chromatography systems evaluated, only Gelman ITLC-SA with 20% NaCl and Gelman ITLC-SG with distilled water correlated with GCS in evaluating free pertechnetate and hydrolyzed reduced 99mTc levels for all IDA radiopharmaceuticals. The miniaturized chromatography procedure, as outlined, is rapid, taking less than 4 min, and can easily be incorporated into the daily quality control program in any nuclear medicine facility.