Studies on the Kinetics of Fungicidal Action: Part I. The Effect of Concentration and Time on the Viability ofPenicillium notatum Spores in Solutions of Phenol

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Abstract
About 98 per cent of Penicillium notatum spores produced germ tubes in liquid nutrient media within 12 hr. Viability was unaffected by storage in distilled water for 20 days at 4°. The sporicidal activity of phenol was measured by determining the percentage of germinated spores before and after contact with phenol solution. Spores were separated on membrane filters from fungicide by rapid filtration, and incubated in Horowitz fluid medium. The rate of death in solutions containing 0.5 to 1.25 per cent phenol followed a first order reaction. The values of the concentration exponent for 50 and 100 per cent mortalities were 12 and 10.5 respectively