Carotene: effect of antioxidant on its determination

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Abstract
Dried grass and lucerne meals were treated with NN[image]-diphenyl-p-phenylenediamine, an antioxidant sometimes used to reduce loss of carotene during storage. Both treated and untreated meals were assayed for carotene immediately by chromatographing a light petroleum extract on aluminium oxide and estimating the extinction of blue light. The antioxidant was found not to interfere in the estimation of carotene although Beachene et al. (1953) had found that it does interfere when magnesium oxide was used for the chroma-tography.

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