Alcohol enzyme of Bact. coli
- 1 September 1940
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 34 (8-9) , 1177-1182
- https://doi.org/10.1042/bj0341177
Abstract
Escherichia coli oxidized alcohol and acetaldehyde. Using the Booth-Green wet-crushing mill alcohol dehydrogenase was obtained soluble and cell-free. The enzyme oxidizes ethyl alcohol in the following system; alcohol dehydrogenase[long dash]coenzyme I[long dash]diaphorase[long dash]methylene blue[long dash]oxygen. Semicarbazide was required as a fixative for the acetaldehyde produced. Acet-aldehyde was identified by means of its 2,4-dinitrophenyl-hydrazone. The enzyme was inhibited by iodoacetate. It is probably identical with the corresponding enzyme of yeast isolated by Negelein and Wulff.This publication has 6 references indexed in Scilit:
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- A wet-crushing mill for micro-organismsBiochemical Journal, 1938
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