METHYLENE BLUE REDUCTION DISK TEST (MRD-TEST) - SIMPLIFIED METHOD FOR IDENTIFICATION OF PSEUDOMONAS SPECIES

  • 1 January 1977
    • journal article
    • research article
    • Vol. 239  (4) , 504-509
Abstract
The identification of Pseudomonas spp. can be improved by testing the utilization of different organic compounds as single sources of carbon. The introduction of this kind of technique into routine laboratory work requires a simplification of the test system. In order to meet these requirements the methyleneblue reduction disc test (M.R.D.-test) was developed. In this test, discs loaded with different organic compounds are introduced into suspensions of the strain examined as single source of carbon. Before, a mineral salts solution containing methyleneblue as a redox indicator was added to these bacterial suspensions. With the help of this method the utilization of a large number of different organic compounds as single sources of carbon can be tested in a way which is neither laborious nor time consuming. The disc once prepared can be stored for some time, single strains can be tested without any difficulty as they are isolated during routine laboratory work.

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