Acetylcholinesterase Activity Values

Abstract
One of the recurrent problems arising in the course of a prospective study is that of changing methods. One problem was to relate older data obtained with the Michel method to that obtained with a newer pH-Stat method. Fresh plasma and washed red cells from five volunteers were inhibited with various amounts of malathion. Cholinesterase activity was determined for each sample by both methods in duplicate. The mean pH-Stat values were regressed on the mean Michel values. The calculated equation for red cel! values was μM/ml/min = 23.15 ΔpH/hr – 5.805; and for plasma values, μM/m//min = 3.26 ΔpH/hr + 0.15. An analysis of variance showed each of these regressions to be highly significant (P < 0.005).

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