DIMETHYL NITROSAMINE‐INDUCED PROLIFERATIVE AND NEOPLASTIC LESIONS IN THE MOUSE LIVER CHARACTERIZED BY HISTOPATHOLOGY AND FLOW CYTOMETRIC DNA MEASUREMENTS

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Abstract
Thirty‐one liver tumours induced in NMRI mice by treatment of the newborn animals with the carcinogen demethyl nitrosamine were examined histopathologically and by flow cytometric determination of nuclear DNA content. The tumours were classified as nodules of hyperplasia, adenomas or hepato‐cellular carcinomas. Single cell suspensions from each tumour were analyzed by flow cytometry. The lesions consisted of cells with stemlines of low ploidy (2c and 4c) as compared to the high ploidy of the surrounding parenchymal cells (4c, 8c and 16c). The hyperplastic nodules contained stemlines of both diploid and tetraploid DNA content, whereas the adenomas and most of the carcinomas were diploid. The possible occurrence of aneuploid cells in the lesions is discussed.