Uptake and release of DNA by lymphoid tissue and cells.
- 1 December 1974
- journal article
- Vol. 27 (6) , 973-87
Abstract
Newly synthesized DNA has been shown to be released by immunized rabbit spleen tissue cultured in vitro. This DNA was mainly doublestranded, showed a large spread in buoyant density, and was in the molecular weight range 50,000–500,000. Rabbit spleen tissue and human peripheral blood lymphocytes in active DNA synthesis, also took up bacterial DNA into their nuclei. After short periods of culture this DNA had a buoyant density of the bacterial DNA employed. Upon prolonged incubation, the DNA was of mammalian density. Evidence was obtained, in the case of the blood lymphocytes, for the appearance in the cells of a DNA of intermediate buoyant density. The possible relevance of the loss and reutilization of DNA to lymphoid cell function is discussed.This publication has 26 references indexed in Scilit:
- In vitro synthesis of DNA spontaneously released by bacteria or frog auriclesBiochimie, 1972
- Bacterial Ribonucleic Acid in the Frog Brain after a Bacterial Peritoneal InfectionScience, 1972
- Agrobacterium tumefaciens RNA in non-tumorous tomato cellsBiochemical and Biophysical Research Communications, 1969
- Fate of exogenous bacterial deoxyribonucleic acids in barley seedlingsJournal of Molecular Biology, 1969
- DNA Uptake by a Mutant Strain of Lymphoma CellsNature, 1968
- Bacterial Nature of Radioactive DNA found in Tomato Plants incubated in the Presence of Bacterial DNA-3HNature, 1968
- Nuclear Uptake of Exogenous DNA by Mammalian Cells in CultureNature, 1968
- Metabolic DNA in Heart and Skeletal Muscle and in the Intestine of MiceNature, 1967
- The uptake of deoxyribonucleic acid released from damaged cells in tissue culturesExperimental Cell Research, 1967
- Uptake of exogenous DNA by mouse embryosExperimental Cell Research, 1967