Determination of Substrate and Product Concentrations in Lactic Acid Bacterial Fermentations by Proton NMR Using the ERETIC Method

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Abstract
A 1H NMR method is described for the determination of the concentration of a chemically diverse group of metabolites over a wide concentration range in fermentation medium. The method employs the use of the ERETIC signal, which produces a pseudo-FID acquired with the FID derived from the sample components. It is shown that this signal, once calibrated against an external standard solution, can be used to determine accurately the concentration of all components present in the medium. The method is rapid, reliable, and applicable to a wide range of biological fluids. Problems such as the invisibility of certain components in the spectrum are overcome by a simple regulation of the pH.