Assessment of the sensitivity of leukaemic cells to cytotoxic drugs by bioluminescence measurement of ATP in cultured cells
- 1 July 1986
- journal article
- research article
- Published by Portland Press Ltd. in Clinical Science
- Vol. 71 (1) , 81-88
- https://doi.org/10.1042/cs0710081
Abstract
1. A short-term test in vitro is described, which can be used to detect resistance to cytostatic agents in leukaemic cells. Leukaemic cell suspensions were incubated with cytostatic agents and the resulting intracellular ATP concentrations were measured by a bioluminescence ATP assay. 2. There was a clear dose-effect relationship in acute leukemia and chronic lymphocytic leukemia cells for drugs used in the treatment of leukemias. A good correlation was found between the ATP content of leukemic cells and cell viability as determined by the trypan blue dye exclusion test. 3. Preliminary individual clinical correlations suggest a correlation between the chemosensitivity in vitro and the response patterns in vivo in leukemia patients. This simple, fast and sensitive method may have application for determination of drug-induced cytotoxicity in leukemic cells in vitro.This publication has 6 references indexed in Scilit:
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