METABOLIC-CLEARANCE OF FUROSEMIDE IN RAT

  • 1 January 1977
    • journal article
    • research article
    • Vol. 200  (1) , 52-57
Abstract
The effect of probenecid on the metabolic clearance of 35S-furosemide was examined in Sprague-Dawley rats by the single injection technique. Metabolic clearance was found to decrease from 1.74 .+-. 0.11 ml/min in animals which received furosemide alone to 1.06 .+-. 0.10 ml/min in animals which received both furosemide and probenecid. Estimation of urine and biliary clearance revealed that approximately 2/3 of the metabolic clearance was due to urinary clearance and 1/3 to biliary clearance, and that both decreased after probenecid administration. TLC studies indicated furosemide was metabolized by both liver and kidney but also secreted in unchanged form in urine, and that the proportion of unaltered furosemide to metabolite in urine was increased by probenecid. Phenobarbital pretreatment had no effect on either hepatic or renal clearance of furosemide, and bile clearance was not altered by acute bilateral nephrectomy.

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