Enumeration of Enterococcus sp. using a modified mE method
Open Access
- 1 June 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 83 (1) , 120-126
- https://doi.org/10.1046/j.1365-2672.1997.00200.x
Abstract
A modified mE medium (mEI) containing the chromogenic substrate indoxyl‐β‐D‐glucoside to detect β‐D‐glucosidase activity was evaluated with respect to specificity and recovery of enterococci from environmental waters. Extending incubation from 24 to 48 h improved enterococci recovery but 77% of the colonies classified as non‐target were confirmed as enterococci. Randomly chosen enterococcal isolates from sewage, exposed in microcosms containing 0·22 μm membrane filtered fresh or estuarine water, exhibited differences in persistence as a function of exposure treatment. Decreasing the concentration of or eliminating indoxyl‐β‐D‐glucoside from mE did not significantly affect recovery of purified isolates.Keywords
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