Vesicular stomatitis virus RNA polymerase can read through the boundary between the leader and N genes in vitro

1 May 1981

journal article
research article
Published by American Society for Microbiology in Journal of Virology

Vol. 38 (2) , 792-796
https://doi.org/10.1128/jvi.38.2.792-796.1981

Abstract

Triphosphorylated in vitro transcripts of vesicular stomatitis virus were selected by Hg Sepharose chromatography using adenosine-5''-O-(3-thiotriphosphate) as an affinity probe. Numerous RNA ranging from < 47 up to several hundred nucleotides in length were detected. Some of these contain the leader RNA covalently linked to transcripts of the N gene. A comparison of the published genomic sequences at the estimated termination sites of several of these RNA reveals some homology with the sequence present at both the end of the leader and polymerase genes.

This publication has 18 references indexed in Scilit:

Unique mode of transcription in vitro by vesicular stomatitis virus
Cell, 1980
Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus
Cell, 1980
3′-Terminal labelling of RNA with T4 RNA ligase
Nature, 1978
Analysis of rna initiated in isolated mouse myeloma nuclei using purine nucleoside 5′[γ-S]triphosphates as affinity probes
Cell, 1978
Complete nucleotide sequence of the leader rna synthesized in vitro by vesicular stomatitis virus
Cell, 1978
In vitro synthesis of a possible precursor RNA by purified vesicular stomatitis virus
Biochemical and Biophysical Research Communications, 1978
A unique RNA species involved in initiation of vesicular stomatitis virus RNA transcription in vitro
Cell, 1976
The 5′ sequences of VSV in vitro transcription product RNA (± SAM)
Biochemical and Biophysical Research Communications, 1976
Messenger RNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus
Cell, 1975
Vesicular stomatitis virus—A new interfering particle, intracellular structures, and virus-specific RNA
Virology, 1970