Immunologic Unresponsiveness in the Adult Guinea Pig

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Abstract
Summary: Sephadex chromatography of commercial HSA results in a low polymer fraction (at least up to pentamer) comprising 12% to 28% of total protein and a nearly homogeneous monomer fraction comprising the remainder. Both fractions and starting material are cleared from the serum of guinea pigs at identical rates, induce unresponsiveness with equal effectiveness, and are immunogenic when incorporated in adjuvant. Heat-aggregated HSA is relatively ineffective as a tolerogen. Heat-aggregated HGG is initially cleared from the serum more rapidly than native HGG. However, it elicits a state of split tolerance in adult guinea pigs with greatly depressed delayed hypersensitivity and hemolytic antibody but with undiminished 7 S γ1 titers. Further evidence is presented documenting a diminution of all immunologic parameters including immune clearance and 7 S γ1 antibody titers in animals suppressed with HSA in saline. These findings are inconsistent with the interpretation that unresponsiveness represents immune deviation.

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