Phosphorylation of chicken gizzard myosin and the Ca2+‐sensitivity of the actin‐activated Mg2+‐ATPase
- 11 July 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 158 (1) , 17-20
- https://doi.org/10.1016/0014-5793(83)80667-x
Abstract
A method is described for the preparation of partially and fully phosphorylated chicken gizzard myosin. When fully phosphorylated it possessed an actin-activated Mg2+-ATPase of similar specific activity to that of mammalian skeletal muscle myosin. The Mg2+-ATPase activity of these preparations was related in a non-linear fashion to increasing phosphorylation of the P light chain. When P light chain phosphorylation occurred during enzymic assay the Mg2+-ATPase activity remained constant. Fully phosphorylated preparations of gizzard myosin possessed an actin-activated Mg2+-ATPase that was not Ca2+-sensitive, whereas the Mg2+-ATPase of partially phosphorylated myosin preparations was Ca2+-sensitive.Keywords
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