D/H Amide Isotope Effect in Model α-Helical Peptides
- 1 November 2002
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 124 (47) , 13994-13995
- https://doi.org/10.1021/ja027740n
Abstract
The contribution of amide related hydrogen bonds to protein stability has recently been evaluated using the “Cm experiment”, which measures the D/H amide isotope effect in proteins. We show here using isolated α-helical peptides that there is a significant effect of denaturant concentration on the measured D/H isotope effect, and that valid comparison of different proteins requires correcting for differences in denaturant (GdmHCl) concentration. Finally our results suggest that H-bonds in an isolated α-helix may contribute more to helix stability because of less strain compared to those in helical proteins and that the buried helical H-bonds in helical proteins are not necessarily energetically more favorable than solvent exposed H-bonds in isolated helices.Keywords
This publication has 11 references indexed in Scilit:
- Understanding protein hydrogen bond formation with kinetic H/D amide isotope effectsNature Structural & Molecular Biology, 2002
- Polar Interactions with Aromatic Side Chains in α-Helical Peptides: Ch···O H-Bonding and Cation−π InteractionsJournal of the American Chemical Society, 2001
- FTIR-Spectroscopy of Multistranded Coiled Coil ProteinsBiochemistry, 1999
- Hydrogen Bonding and Equilibrium Protium−Deuterium Fractionation Factors in the Immunoglobulin G Binding Domain of Protein GBiochemistry, 1999
- Trifluoroethanol Promotes Helix Formation by Destabilizing Backbone Exposure: Desolvation Rather than Native Hydrogen Bonding Defines the Kinetic Pathway of Dimeric Coiled Coil FoldingBiochemistry, 1998
- Infrared Amide I‘ Band of the Coiled CoilBiochemistry, 1996
- Periodicity of amide proton exchange rates in a coiled-coil leucine zipper peptideBiochemistry, 1991
- Unusually stable helix formation in short alanine-based peptides.Proceedings of the National Academy of Sciences, 1989
- Structures and isotopic fractionation factors of complexes, A1HA2-Journal of the American Chemical Society, 1980
- Characterization and molecular structure of [(PPh3)RhC2B9H11]2, a phosphinorhodacarborane dimer containing rhodium-hydrogen-boron bridgesJournal of the American Chemical Society, 1978