The volume of uterine fluid in 'implanting' and 'delayed implanting' mice

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Abstract
A variation of the dye dilution technique was used to determine the volume of uterine fluid in implanting and delayed implanting mice. The method involves rinsing Krebs-Ringer-bicarbonate buffer containing [methyl-14C]methylated-BSA [bovine serum albumin] through the uterine lumen and using the resulting decrease in concentration of the BSA to calculate the volume of uterine fluid. The volume of uterine fluid was essentially the same in implanting and delayed implanting mice (i.e., 300-400 nl/pair of uterine horns). The total amounts of a substance recovered by rinsing the uteri thus provide an estimate of relative concentrations in situ.