The uptake of Glucose and gluconate by Pseudomonas putida

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Abstract
Summary The uptake of glucose and gluconate is under inductive control inPseudomonas putida. Glucose, gluconate, and 2-ketogluconate were each good nutritional inducers of these transport abilities. Glucose and gluconate uptake obeyed saturation kinetics: the apparent Km for glucose was 6mm and that for gluconate was 0.5mm. Therefore, transport of both substrates appears to be mediated by enzyme-like carriers. Glucose and gluconate are parallel inhibitors for their uptake. Strains selected for their inability to transport glucose were found to be deficient in gluconate uptake. The reverse was also true: mutations affecting gluconate entry also blocked the uptake of glucose. These results demonstrate that a common carrier is involved in the uptake of both glucose and gluconate byP. putida cells.