Quantitative RT‐PCR analysis of pacifastin‐related precursor transcripts during the reproductive cycle of solitarious and gregarious desert locusts
- 14 March 2008
- journal article
- Published by Wiley in Insect Molecular Biology
- Vol. 17 (2) , 137-145
- https://doi.org/10.1111/j.1365-2583.2008.00793.x
Abstract
In locusts, little is known about the physiological and biochemical mechanisms regulating complex processes, such as reproduction and phase transition. The pacifastin family constitutes a family of peptidic inhibitors of serine proteases that are considered to be important regulators of several physiological processes in arthropods. We have performed a detailed transcript profiling analysis of two pacifastin‐related peptide precursors, SGPP‐2 and SGPP‐4, during the reproductive cycle of adult desert locusts (Schistocerca gregaria). This quantitative real‐time (RT)‐PCR analysis revealed a temporal regulation of both transcripts, which is paralleled by several events that occur during the reproductive cycle of adult locusts. The observed temporal transcript profiles display a strong tissue‐, gender‐ and phase‐dependence. In addition, a partial regregarization experiment suggests that both transcript levels are regulated during phase transition and can be employed as molecular markers of the gregarization process.Keywords
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