Production of emetic alkaloid by in vitro culture of cephaelis ipecacuanha A. Richard
- 1 June 1988
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 7 (4) , 278-280
- https://doi.org/10.1007/bf00272542
Abstract
Callus and adventitious roots were induced on leaf segments from shoot culture of Cephaelis ipecacuanha A. Richard on Murashige-Skoog medium containing 2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, 1-naphthaleneacetic acid and kinetin. The contents of emetic alkaloids in calli, roots and root suspension cultures were quantified by HPLC. Roots cultured in solid and liquid Murashige-Skoog media yielded emetine and cephaeline. The amount of the two alkaloids in the root suspension culture was very similar to that of roots from ipecac mother plant grown in a greenhouse. In contrast, calli subcultured on Murashige-Skoog media containing combinations of 2,4-dichlorophenoxyacetic acid and kinetin produced only trace amounts of emetic alkaloids.Keywords
This publication has 4 references indexed in Scilit:
- Alkaloid production by hairy root cultures in Atropa belladonnaPlant Cell Reports, 1986
- Cell Suspension Cultures ofCinchona ledgeriana; II. The Effect of a Range of Auxins and Cytokinins on the Production of Quinoline AlkaloidsPlanta Medica, 1986
- Cell Suspension Cultures ofCinchona ledgeriana; I. Growth and Quinoline Alkaloid ProductionPlanta Medica, 1986
- Alkaloid production in cultured roots of three species of DuboisiaPhytochemistry, 1985