1H NMR INVESTIGATION OF COMPETITIVE BINDING OF SULFUR-CONTAINING PEPTIDES AND GUANOSINE 5′-MONOPHOSPHATE TO A MONOFUNCTIONAL PLATINUM(II) COMPLEX

Abstract
The competitive binding of N-acetylglycyl-D-L-methionine (AcGly-Met) and guanosine-5′-monophosphate (5′-GMP) to the monofunctional [Pt(dien)Cl]+ complex has been studied by1H NMR spectroscopy. Results have shown that in the initial stages of the reaction the platinum(II) complex only reacts with the methionine containing peptide, and the second step of this reaction is the very slow intermoiecular displacement of the S-bound thioether ligand with the N7 atom of the guanine base of 5′-GMP. Comparative reactions of glutathione have also been investigated. The obtained results have been analyzed in relation to the antitumour activity and toxicity of platinum complexes.

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