Functional significance of cumulus expansion in the mouse: Roles for the preovulatory synthesis of hyaluronic acid within the cumulus mass
- 1 January 1993
- journal article
- research article
- Published by Wiley in Molecular Reproduction and Development
- Vol. 34 (1) , 87-93
- https://doi.org/10.1002/mrd.1080340114
Abstract
Gonadotropin‐stimulated expansion of the mouse cumulus oocyte complex (COC) in vitro, measured with a quantitative videographic method, is comparable to that observed to occur in vivo when medium is supplemented with porcine follicle stimulating hormone (pFSH), 10% fetal bovine serum (FBS), and 2.5 mM glucosamine or optimal concentrations of glutamine and glucose. In the absence of glucosamine, the volumetric expansion of COCs in vitro is never more than 25% of that occurring in its presence. The addition of 6‐diazo‐5‐oxo‐1‐norleucine (DON), an inhibitor of glucosamine synthesis to medium supplemented with glutamine and glucose, completely inhibits cumulus expansion in vitro. This system was utilized to examine the relationship between cumulus expansion and fertilization rates, and the maintenance of fertilizability in culture. Successful fertilization (as determined by development to the 2‐cell stage) was correlated with the quantity and quality of the expanded cumulus mass, and conversely, the spontaneous loss or mechanical removal of the cumulus was correlated with a loss of fertilizability following additional incubation in culture medium. In addition, the i.p. injection of DON inhibited cumulus expansion within the intact follicle and suppressed ovulation.Keywords
This publication has 28 references indexed in Scilit:
- Hyaluronic acid synthesis and gap junction endocytosis are necessary for normal expansion of the cumulus massMolecular Reproduction and Development, 1990
- Hyaluronidase removal of the cumulus oophorus increases in vitro fertilizationJournal of Assisted Reproduction and Genetics, 1988
- Chromosome Configurations and Time Sequence of the First Meiotic Division in Bovine Oocytes Matured in Vitro1Biology of Reproduction, 1988
- Prevention of Spontaneous Degeneration of Mouse Oocytes in Culture by Ovarian Glycosaminoglycans1Biology of Reproduction, 1987
- In Vitro Fertilization: State of the ArtSeminars in Reproductive Medicine, 1984
- The developmental capacity of mouse oocytes that matured spontaneously in vitro is normalDevelopmental Biology, 1984
- Effects of egg aging on in vitro fertilization and first cleavage division in the hamsterGamete Research, 1983
- The relationship between cumulus cell-oocyte coupling, oocyte meiotic maturation, and cumulus expansionDevelopmental Biology, 1982
- Maturational Effects of Gonadotropins on the Cumulus-Oocyte Complex of the RatBiology of Reproduction, 1979
- The AmidotransferasesPublished by Wiley ,1973