A STUDY OF THE EFFECT OF SEVERAL EXPERIMENTAL CONDITIONS ON THE INTERMEDIN CONTENT AND CYTOCHEMICAL REACTIONS OF THE INTERMEDIATE LOBE OF THE FROG (RANA PIPIENS)

Publisher Website
Google Scholar
Abstract
In addition to glycogen, 2 classes of intracellular granules (A and B) were found in the intermediate lobe of the frog (Rana pipiens). Type A granules were numerous, small, and gave positive reactions for carbohydrates and proteins. Type B granules were less abundant, larger and gave a positive reaction for phospholipid. Large intracellular vesicles accumulating in the region of the transverse vein gave positive reactions for carbohydrates, proteins and phospholipids. Injection of purified intermedin had no detectable effect on the pars intermedia cells. Thyroidectomy also produced no effect on pars intermedia cytology, chromatic physiology or activity of the frogs. Pituitaries contained a maximum of 2000 Lerner units of intermedin. By calculation this might represent about O.l[mu]g intermedin in the intermediate lobe. However, no evidence was found to implicate intermedin with either granules of type A or B or with the vesicles.

This publication has 2 references indexed in Scilit: