INHIBITION OF CYCLIC-AMP ACCUMULATION IN DOG HEART WASHED PARTICLE PREPARATIONS BY APROTININE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 28  (2) , 255-266
Abstract
The effect of aprotinine, a natural nonspecific protease inhibitor, on cyclic[c]AMP accumulation in a dog heart washed particle preparation was studied. Aprotinine inhibited GTP-stimulated cAMP accumulation at concentrations equal to or higher than 40 KIU[kallikrein-inhibiting units]/ml (8 .times. 10-7 M). Aprotinine inhibited epinephrine (10-4 M)-stimulated cAMP accumulation to a greater degree than fluoride (10-2 M)-stimulated activity. Trypsin stimulated cAMP accumulation at low concentrations (0.5-2.5 .mu.g/ml) and inhibited at higher concentrations. Aprotinine at low concentrations (4 KIU/ml) inhibited the trypsin (1 .mu.g/ml) stimulation of cAMP accumulation. Aprotinine may act on the regulator rather than on the catalytic subunit of adenylate cyclase and its mechanism of action is not due only to its antiprotease activity.

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