Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae
- 1 February 1980
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 27 (2) , 657-666
- https://doi.org/10.1128/iai.27.2.657-666.1980
Abstract
Two Escherichia coli O6:K2:H1 strains, C1212 and C1214, isolated from urinary tract infections, were compared for their capacity to adhere to various cells. After growth on solid medium, only C1212 bacteria agglutinate human erythrocytes and attach to urinary epithelial cells. Both of these reactions are mannose resistant. In contrast, C1214 bacteria cause a mannose-sensitive agglutination of guinea pig erythrocytes, show a mannose-sensitive attachment to buccal epithelial cells, and attach to urinary mucus. Immunoelectron microscopy revealed that C1214 bacteria possess type 1 fimbriae (mannose sensitive), which are not present in C1212 bacteria when this strain is grown on solid medium. The fimbriae of C1212 (mannose resistant) were also demonstrated by immunoelectron microscopy. We call these fimbriae demonstrated in C1212 the E. coli F7 antigen. Urinary mucus, and probably mucous material elsewhere, may function as a trap for Enterobacteriaceae with type 1 fimbriae by the specific adherence of such bacteria. We consider this a nonimmune resistance mechanism against disease caused by Enterobacteriaceae.This publication has 52 references indexed in Scilit:
- Type I Escherichia coli pili: characterization of binding to monkey kidney cells.The Journal of Experimental Medicine, 1977
- Haemagglutinating and Adhesive Properties Associated with the K99 Antigen of Bovine Strains of Escherichia coliJournal of General Microbiology, 1976
- THE ESTABLISHMENT OF K99, A THERMOLABILE, TRANSMISSIBLE ESCHERICHIA COLI K ANTIGEN, PREVIOUSLY CALLED “Kco”, POSSESSED BY CALF AND LAMB ENTEROPATHOGENIC STRAINSActa Pathologica Microbiologica Scandinavica Section B Microbiology, 1975
- The Association of K88 Antigen with Haemagglutinating Activity in Porcine Strains of Escherichia coliJournal of General Microbiology, 1974
- Intermediate Gel in Crossed and in Fused Rocket ImmunoelectrophoresisScandinavian Journal of Immunology, 1973
- General Remarks on Principles, Equipment, Reagents and ProceduresScandinavian Journal of Immunology, 1973
- Observations On The Pathogenic Properties Of The K88, Hly And Ent Plasmids Of Escherichia Coli With Particular Reference To Porcine DiarrhoeaJournal of Medical Microbiology, 1971
- Simple Immunological Labelling Method for Electron Microscopy and its Application to the Study of Filamentous Appendages of BacteriaNature, 1967
- Electrophoresis and phage susceptibility studies on a filament-producing variant of the E. coli B bacteriumBiochimica et Biophysica Acta, 1954
- Electron microscopical observations on bacterial cytology II. A study of flagellationBiochimica et Biophysica Acta, 1950