Determination of Thiocyanate in Serum with the Pyridine-Benzidine Reaction

Publisher Website
Google Scholar
Abstract
Considerable errors can occur in the determination of thiocyanate in blood serum if the deproteinization with trichloracetic acid is not carried out under certain conditions. The error is due to the binding of thiocyanate ions to the proteins. Minimum losses take place if 1 volume of serum is diluted with 1 volume of distilled water, and the proteins precipitated with 1 volume of 50% (w/v) trichloracetic acid in water. The pyridine-benzidine method, as so far employed, gives in certain cases considerable non-specific color, which makes exact measurements impossible. A "blank" is introduced to counteract this disadvantage. Thiocyanate is precipitated as its silver salt and excess Ag+ ions are removed with NaCl. Increased specificity is thereby obtained.

This publication has 4 references indexed in Scilit: