Partial substitution of serum in hematopoietic cell line media by synthetic polymers.
- 1 June 1970
- journal article
- Vol. 19 (6) , 906-10
Abstract
Several synthetic polymers (hydroxyethyl starch, sodium carboxymethyl cellulose, polyvinylpyrrolidone) markedly improved the growth of three human lymphocyte cell lines [Roswell Park Memorial Institute (RPMI) 1348, 1788, and 8098]. Growth was stimulated when each of these polymers was added to RPMI 1640 medium supplemented with only 2% fetal bovine serum. Dextran T-40, T-70, and T-110 varied in their effect on the growth of these cell lines. Dextran T-250 and Haemaccel did not improve cell yields when partially substituted for the serum. The successful partial substitution of polymers for serum was specific for individual cell lines.This publication has 7 references indexed in Scilit:
- Lymphoblastoid cell lines from normal persons and those with nonmalignant diseasesJournal of Surgical Research, 1969
- Methylcellulose effect on cell proliferation and glucose utilization in chemically defined medium in large stationary culturesBiotechnology & Bioengineering, 1969
- Replacement of Plasma by High Molecular Weight SubstancesAngewandte Chemie International Edition in English, 1968
- MAMMALIAN CELLS IN CHEMICALLY DEFINED MEDIA IN SUSPENSION CULTURES*Annals of the New York Academy of Sciences, 1966
- Air-thermostated chamber with proportional temperature control for tissue culture applicationJournal of Applied Physiology, 1962
- The mechanism of cell binding and cell-sheet formation in L strain fibroblastsExperimental Cell Research, 1960
- The Action of Vital Stains Belonging to the Benzidine GroupScience, 1914