Chromosome Contraction byo-Isopropyl-N-Phenylcarbamate (IPC)

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Abstract
A selective herbicide IPC (o-isopropyl-N-phenlycarbamate) causes contraction of chromosomes in the prophase, metaphase, and anaphase stages of mitosis in cells of treated root tips. Effective concentrations in aqueous solution lie between 2.5 and 50 ppm, and effective times between 1 and 4 hr, depending upon the species of plant. A suggested starting combination is 10 ppm for 2 hr. This compound is effective in causing contraction of chromosomes in a wide range of plant species, as well as enhancing separation in acetocarmine and aceto-orcein squashes in many cases. Possibly, it may produce similar results in species which have been found to be unaffected by colchicine, 8-hydroxyquinoline, p-dichlorobenzene, and other commonly used chemicals.