Continuous culture of bacteriophage Q-beta using a cellstat with a bubble wall-growth scraper
- 1 June 1987
- journal article
- letter
- Published by AIP Publishing in Review of Scientific Instruments
- Vol. 58 (6) , 1109-1111
- https://doi.org/10.1063/1.1139615
Abstract
It has been shown that a virulent phage Qβ can be cultured continuously in a cellstat which was developed to culture a bacteriophage fd for the study of molecular evolution. A cloned disadvantageous mutant was cultured to obtain a biological relaxation curve to selection/mutation equilibrium. A new technique for scraping off the wall growth by air bubbles is introduced. The scraper worked well in short‐time cultivation of Qβ. It has various advantages of a closed system.Keywords
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