The Structure of the Murine Calcium Channel γ-Subunit Gene and Protein
- 1 January 1998
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry
- Vol. 379 (1) , 45-50
- https://doi.org/10.1515/bchm.1998.379.1.45
Abstract
The gamma-subunit of voltage-gated calcium channels is a membrane protein that is associated with the skeletal muscle type of voltage gated calcium channels. Using a subunit-specific polyclonal antibody, the gamma-protein was detected in mouse skeletal muscle but not in brain, where at least five additional types of voltage-gated calcium channels are expressed. Murine genomic clones containing the full coding sequence of the gamma-subunit were isolated, the exons were mapped and sequenced. The murine gamma-subunit is encoded by a single copy gene containing 4 translated exons which are distributed over approximately 14 kilobases of DNA. The intron placement within the mouse gene correlates with the previously revealed organization of the human gamma-subunit gene, although the primary structures of the gamma-subunits are only moderately conserved between the murine, human, rat and rabbit proteins (75% identity).Keywords
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