Immunological studies on the D‐1 and D‐2 proteins of photosystem II preparations from the thermophilic cyanobacterium, Synechococcus sp
- 6 June 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 233 (1) , 1-6
- https://doi.org/10.1016/0014-5793(88)81344-9
Abstract
Immunoblotting of Synechococcus photosystem II preparations with site‐directed antisera against the spinach D‐2 protein and an antiserum against a large polypeptide segment of the spinach D‐1 protein showed (i) that the 28 and 31 kDa polypeptides of the cyanobacterial photosystem II core complex are equivalent to the D‐1 and D‐2 proteins of higher plants and algae and (ii) that the β‐octylglucoside treatment of the photosystem II preparation diminishes band intensities of the 28 and 31 kDa proteins in Coomassie blue‐stained gels mainly by increasing the amounts of two proteins which migrate as a dimer of D1 or D2 or its aggregate, and of proteolytic digestion products.Keywords
This publication has 17 references indexed in Scilit:
- Characterisation of a PS II reaction centre isolated from the chloroplasts ofPisum sativumFEBS Letters, 1987
- Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b -559Proceedings of the National Academy of Sciences, 1987
- Herbicide and plastoquinone-binding proteins of Photosystem II reaction center complexes from the thermophilic cyanobacterium, Synechococcus sp.Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1986
- Photosystem II particles largely depleted in the two intrinsic polypeptides in the 30 kDa region from Synechococcus sp.FEBS Letters, 1986
- PROTEIN‐PIGMENTS AND PHOTOSYSTEM II REACTION CENTERPhotochemistry and Photobiology, 1985
- Inhibition by ethylenediamine tetraacetate and restoration by Mn2+ and Ca2+ of oxygen-evolving activity in Photosystem II preparation from the thermophilic cyanobacterium, Synechococcus spBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- Nucleotide and deduced polypeptide sequences of the photosynthetic reaction-center, B870 antenna, and flanking polypeptides from R. capsulataCell, 1984
- A photoactive Photosystem-II reaction-center complex lacking a chlorophyll-binding 40 kilodalton subunit from the thermophilic cyanobacterium Synechococcus sp.Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1984
- Polypeptide composition of a Photosystem II core complex. Presence of a herbicide-binding proteinBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1983
- Photoaffinity labeling of an herbicide receptor protein in chloroplast membranesProceedings of the National Academy of Sciences, 1981