Isolation of hatching gland cells from the teleost, Oryzias latipes, by centrifugation through percoll

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Abstract
Hatching gland cells (HGCs) of the teleost, Oryzias latipes, have been isolated. The optimum condition is mechanical dissociation of the buccal tissues in Ca‐free saline containing 15% bovine serum albumin (BSA). The cell suspension is fractionated by centrifugation through 30–33.3% Percoll TM containing 5% BSA. The isolated HGCs are washed in 5% BSA. BSA in the latter two solutions can be omitted when a swing‐out rotor is used. The recovery of total and viable HGCs was about 74% and 45%, respectively. The purity was 75–85%. Cellular integrity was shown by pseudopodial movements of the cultured cells.