Electron transport system activity in Daphnia and crayfish
- 1 May 1977
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Zoology
- Vol. 55 (5) , 847-854
- https://doi.org/10.1139/z77-110
Abstract
The activity of the electron transport system (ETS) in D. magna and the crayfish Orconectes propinquus was estimated by measuring NADH-, succinate- and NADPH-cytochrome c reductase. The activity of the total ETS was measured by following NADH oxidation, and by measuring the rate of cytochrome c reduction upon addition of cyanide after a steady state between cytochrome c reductase (EC 1.6.99.3) and cytochrome oxidase (EC 1.9.3.1) had been reached. ETS activity was highest in crayfish gill when NADH or succinate was used as substrate, and highest in crayfish hepatopancreas when NADPH was used. Km for NADH, NADPH and cytochrome c were comparable with some of the lower values reported for mammalian systems. The usefulness of the ETS assay in the study of toxic substances is discussed.Keywords
This publication has 5 references indexed in Scilit:
- Effect of delta9-tetrahydrocannabinol on mitochondrial NADH-oxidase activity.Journal of Biological Chemistry, 1976
- The relationship of cobaltous chloride-induced alterations of hepatic microsomal enzymes to altered carbon tetrachloride hepatotoxicityToxicology and Applied Pharmacology, 1976
- Effect of chlorinated benzenes on the metabolism of foreign organic compoundsToxicology and Applied Pharmacology, 1976
- Cytochrome P-450 and adrenodoxin in adrenal mitochondrial fractions of several lower vertebratesComparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1976
- Cadmium inhibition of hepatic drug metabolism in the ratToxicology and Applied Pharmacology, 1974