Studies on Laccases of Lacquer Trees
- 1 September 1961
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 50 (3) , 264-272
- https://doi.org/10.1093/oxfordjournals.jbchem.a127442
Abstract
The properties of purified laccases from Japanse (Rhus vernifera) and Indochina lacquers (R. succedanea) are presented:Cu, 0.23-0.26 and 0.22-0.24%; N, 10.1 and 8.8%; mol. wt., 141,000 and 130,000; absorption bands of the oxidized form, 617 and 607 m[mu]; a complete heat inactivation at 50 and 70[degree]C; QO2 for hydroquinone, 8-9x103 and 7.5-8.5 x 104; opt. pH, 7.5-7.6 and 6.4 and 6.6; Km for hydroquinone, 0.3 and 0.019 [image]; Km for catechol, 0.045 and 0.015 [image], respectively. The Cu content is calculated as 5-6 atoms/mol. with both lac cases.Keywords
This publication has 3 references indexed in Scilit:
- Purification and physico-chemical properties of laccaseBiochimica et Biophysica Acta, 1958
- The determination of glucosamine and galactosamineBiochemical Journal, 1955
- LXIII.—Chemistry of lacquer (Urushi). Part I. Communication from the Chemical Society of TokioJournal of the Chemical Society, Transactions, 1883