Oxygen uptake, ATPase activity, and superoxide dismutase activity in isolated rat liver mitochondria are not influenced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate
- 1 January 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 4 (5) , 509-512
- https://doi.org/10.1093/carcin/4.5.509
Abstract
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) influences neither the state 3 nor the state 4 respiration in rat liver mitochondria. The respiratory control and ADP/O ratio were also unaffected by TPA. The oligomycin-sensitive ATPase activity in submitochondrial particles remained unaltered upon TPA addition, whereas the NADH oxidase activity was slightly inhibited at a very high concentration of TPA (15% decrease at 17 .mu.M TPA). The activity of the superoxide dismutase located to the mitochondria was insensitive to the tumor promoter, and no change in the rate of H2O2 production was found on TPA treatment in vitro. The mitochondrion is not a likely candidate for the site of action of the tumor promoter.This publication has 2 references indexed in Scilit:
- Diaplacental initiation of NMRI mice with 7, 12-dimethylbenz[a]anthracene during gestation days 6–20 and postnatal treatment of the F1-generation with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate: tumor incidence in organs other than the skinCarcinogenesis: Integrative Cancer Research, 1981
- Inhibition of mitochondrial oxidative phosporylation by 4-deoxyphorbol triester, a poisonous constituent of the latex sap of Euphorbia biglandulosa DesfToxicon, 1980