Medullary tissue culture of the red alga Chondrus crispus

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Abstract
Techniques were developed for the isolation and axenic culture of medullary tissue explants from female gametophytic fronds of Chondrus crispus Stackh. When a strain with narrow thick branches was grown in vitro in a special, enriched seawater medium with added growth regulators, the colourless medullary tissue explants (2-mm cubes) produced fronds exhibiting the normal morphology of the parent plant.

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