The Importance of Careful Blood Processing in Isolation of Cell‐Free DNA
- 5 September 2006
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 1075 (1) , 313-317
- https://doi.org/10.1196/annals.1368.042
Abstract
Abstract: In healthy individuals, the source of cell‐free plasma DNA is predominantly apoptotic, whereas, increased plasma DNA integrity is seen in cancer patients. Therefore, it is important to carefully isolate absolutely “cell‐free” plasma DNA. Plasma DNA from 30 healthy females was analyzed using 4 PCR amplicons of increasing size, comparing standard blood processing with additional centrifugation steps prior to DNA extraction. Cellular DNA contamination, indicated by positive amplicons >300 bp was eliminated only after the extra centrifugation step. This highlights the importance of careful processing in preparation of cell‐free plasma DNA as a tool for cancer detection and we recommend the use of a microcentrifuge spin, prior to DNA extraction.Keywords
This publication has 6 references indexed in Scilit:
- Effects of Blood-Processing Protocols on Fetal and Total DNA Quantification in Maternal PlasmaClinical Chemistry, 2001
- Reproducibility in the Quantification of mRNA Levels by RT-PCR-ELISA and RT Competitive-PCR-ELISABioTechniques, 1998
- Microsatellite alterations in plasma DNA of small cell lung cancer patientsNature Medicine, 1996
- Microsatellite alterations in serum DNA of head and neck cancer patientsNature Medicine, 1996
- The Occurrence of Single-Stranded DNA in the Serum of Patients with Systemic Lupus Erythematosus and Other DiseasesJournal of Clinical Investigation, 1973