Matrix‐assisted laser desorption mass spectrometry of cytochromes P450
- 1 January 1993
- journal article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 7 (1) , 16-19
- https://doi.org/10.1002/rcm.1290070105
Abstract
Matrix‐assisted laser desorption ionization on a time‐of‐flight (MALDI/TOF) mass spectrometer provides a rapid and accurate method for molecular weight determination of hydrophobic cytochrome P450 proteins (P450s). A mass accuracy of 0.075% was achieved by analysis of dialyzed P450 2B1 using bovine serum albumin (BSA) as an internal standard. The measured mass of cytochorme P450 2B2 with MALDI/TOF resulted in an average molecular weight which was higher than reported values. Immobilization procedures proved extremely effective on samples containing high concentrations of phosphate buffers.Keywords
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