CONTINUOUS MONITORING OF FREE FATTY-ACID RELEASE FROM ADIPOCYTES BY PH-STAT TITRATION

  • 1 January 1979
    • journal article
    • research article
    • Vol. 20  (4) , 557-560
Abstract
A method for direct and continuous monitoring of free fatty acid release in adipocyte suspensions is described. Using a pH-stat apparatus, the protons from the released free fatty acids are continuously titrated and the accumulated amount of OH- added is monitored on a recorder against time, the slope indicating the rate of free fatty acid release. Since pH is kept constant, an incubation medium with a low buffering capacity can be used, which gives the method a high sensitivity. Under the conditions described, free fatty acid release from 5% of maximal norepinephrine stimulation of rat adipocytes can be accurately measured and the kinetics followed over extended periods of time.