A New Method of obtaining Cultures from Single Bacterial Cells

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Abstract
While investigating variations in agglutinability, occurring among certain strains of paratyphoid bacilli, it became desirable to obtain cultures derived from single bacterial cells. The technique at first adopted was that devised by Mutch (1919), but the results obtained were not satisfactory. Certain modifications of this method were attempted, but were no more successful. The main difficulty, and one which appears to us to have been far too little emphasised in all the methods hitherto described, has been encountered in obtaining satisfactory conditions for the microscopical observation of the bacillary suspensions employed. The difficulty of accurately observing hanging-drop preparations, and still more of controlling manipulation of them by microscopical observation, would seem to us to have been insufficiently realised; and the ease with which faulty interpretation of microscopical appearances may introduce fatal errors into work of this kind has not, perhaps, been fully taken into account.

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