Formation and resolution of recombination intermediates by E. coliRecA and RuvC proteins
- 1 December 1991
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 354 (6354) , 506-510
- https://doi.org/10.1038/354506a0
Abstract
The recombination of DNA molecules has been reconstituted in vitrousing two purified enzymes from Escherichia coli. RecA protein catalyses homologous pairing and strand exchange reactions to form intermediate DNA structures that are acted upon by RuvC. The newly identified RuvC protein resolves the intermediates by specific endonucleolytic cleavage to produce recombinant DNA molecules.Keywords
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