Fish testicular 11β‐hydroxylase : cDNA cloning and mRNA expression during spermatogenesis
- 18 November 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 397 (2-3) , 250-252
- https://doi.org/10.1016/s0014-5793(96)01187-8
Abstract
We isolated and characterized a cDNA encoding testicular 11β‐hydroxylase, cytochrome P450(11β) from the Japanese eel (Anguilla japonica) testis. The cDNA contains an open reading frame that encodes a protein of 511 amino acids. The predicted amino acid sequence shares 38–48% homology with those of adrenal P450(11β) from mammals and frog. Transient expression in COS 1 cells confirmed that the protein encoded by this cDNA had P450(11β) activity. Northern blotting revealed a single 1.8 kb long transcript of P450(11β). This transcript was not found in immature eel testes prior to an injection with human chorionic gonadotropin (hCG), but it was present in eel testes after hCG injection.Keywords
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