Mismatch Tolerance by DNA Polymerase Pol4 in the Course of Nonhomologous End Joining in Saccharomyces cerevisiae
- 1 April 2006
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 172 (4) , 2689-2694
- https://doi.org/10.1534/genetics.105.053512
Abstract
In yeast, the nonhomologous end joining pathway (NHEJ) mobilizes the DNA polymerase Pol4 to repair DNA double-strand breaks when gap filling is required prior to ligation. Using telomere–telomere fusions caused by loss of the telomeric protein Rap1 and double-strand break repair on transformed DNA as assays for NHEJ between fully uncohesive ends, we show that Pol4 is able to extend a 3′-end whose last bases are mismatched, i.e., mispaired or unpaired, to the template strand.Keywords
This publication has 36 references indexed in Scilit:
- Capture of Extranuclear DNA at Fission Yeast Double-Strand BreaksGenetics, 2005
- The Mre11/Rad50/Xrs2 complex and non-homologous end-joining of incompatible ends in S. cerevisiaeDNA Repair, 2005
- A Gradient of Template Dependence Defines Distinct Biological Roles for Family X Polymerases in Nonhomologous End JoiningMolecular Cell, 2005
- Biochemical Properties of Saccharomyces cerevisiae DNA Polymerase IVJournal of Biological Chemistry, 2005
- Non-homologous end joining dependency of γ-irradiation-induced adaptive frameshift mutation formation in cell cycle-arrested yeast cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2004
- Microhomology-Dependent End Joining and Repair of Transposon-Induced DNA Hairpins by Host Factors in Saccharomyces cerevisiaeMolecular and Cellular Biology, 2004
- Indecent Exposure: When Telomeres Become UncappedMolecular Cell, 2004
- Non-homologous end-joining: bacteria join the chromosome breakdanceTrends in Biochemical Sciences, 2003
- Transient Stability of DNA Ends Allows Nonhomologous End Joining to Precede Homologous RecombinationMolecular Cell, 2002
- Association of DNA Polymerase μ (pol μ) with Ku and Ligase IV: Role for pol μ in End-Joining Double-Strand Break RepairMolecular and Cellular Biology, 2002