Mouse Immunoglobulin Subclasses: Cyanogen Bromide Fragments and Partial Sequence of a gamma1 Chain
Open Access
- 1 August 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 56 (2) , 503-519
- https://doi.org/10.1111/j.1432-1033.1975.tb02256.x
Abstract
The purification and characterisation of all the cyanogen bromide fragments of MOPC 21 heavy (γ1) chain is described. The ten BrCN fragments account for the whole chain. Four of these fragments have been used to establish the sequence of the C-terminal stretch (138 residues) that includes the entire CH3 and a part of the CH2 homology regions. A comparison of this sequence with homologous sequences is presented. Mouse γ1 and γ2 proteins differ much more than the γ subclasses in humans and in other species. The comparison further suggests that the four human γ subclasses and mouse γ1 have a common ancestor which differs from the mouse γ2 ancestor. Unlike other subclasses, mouse γ1 and γ2 genes have diverged before speciation.Keywords
This publication has 41 references indexed in Scilit:
- Primary structure of the CH3 homology region from guinea pig IgG2 antibodiesBiochemistry, 1974
- Primary structure of the CH2 homology region from guinea pig IgG2 antibodiesBiochemistry, 1974
- Determination of the Primary Structure of a Mouse IgG2a Immunoglobulin: Amino‐Acid Sequence of the Fc FragmentEuropean Journal of Biochemistry, 1974
- Spontaneous mutation in tissue culture — chemical nature of variant immunoglobulin from mutant clones of MOPC 21FEBS Letters, 1973
- Solid‐phase edman degradation: Attachment of carboxyl‐terminal homoserine peptides to an insoluble resinFEBS Letters, 1973
- Aminopropyl glass and its p‐phenylene diisothiocyanate derivative, a new support in solid‐phase Edman degradation of peptides and proteinsFEBS Letters, 1973
- Solid‐phase Edman degradation. The use of p‐phenyl diisothiocyanate to attach lysine‐ and arginine‐containing peptides to insoluble resinsFEBS Letters, 1972
- Variability of Interchain Binding of Immunoglobulins: Interchain Bridges of Mouse IgG2a and IgG2bNature, 1970
- Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gelsBiochemical and Biophysical Research Communications, 1967
- Halogenation of tyrosine during acid hydrolysisBiochimica et Biophysica Acta, 1963