1H‐NMR structural determination of the N‐linked carbohydrate chains on glycopeptides obtained from the bean lectin phytohemagglutinin

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Abstract
Phytohemagglutinin, the lectin of the common bean Phaseolus vulgaris, is a N‐linked glycoprotein with one high‐mannose‐type and one xylose‐containing oligosaccharide side chain per polypeptide. The high‐mannose‐type glycan is attached to Asn12 and the complex‐type glycan to Asn60 [Sturm, A. & Chrispeels, M. J. (1986) Plant Physiol. 81, 320–322]. The structures of the oligosaccharides were elucidated from two glycopeptides obtained from the lectin by Pronase digestion, affinity chromatography on concanavalin‐A–Sepharose and gel‐filtration chromatography on a column of BioGel P‐4. The N‐linked glycan structures were investigated by 500‐MHz 1H‐NMR spectroscopy and were established to be: image