Growth and substrate consumption of Nitrobacter agilis cells immobilized in carrageenan: Part 2. Model evaluation
- 1 July 1991
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 38 (3) , 232-240
- https://doi.org/10.1002/bit.260380304
Abstract
A dynamic model that predicts substrate and biomass concentration profiles across gel beads and from that the overall substrate consumption rate by the gel beads containing growing cells was evaluated with immobilized Nitrobacter agilis cells in an airlift loop reactor with oxygen as the limiting substrate. The model predictions agreed well with the observed oxygen consumption rates at three different liquid phase oxygen concentrations. Image analysis showed that 90% of the immobilized cells after 42 days of cultivation was situated in the outer shells in a film of 140 μm, while the bead radius was about 1 mm. The maximum biomass concentration in the outmost film of 56 μm was 11 kg · m−3 gel.Keywords
This publication has 44 references indexed in Scilit:
- Scanning electron microscopy of immobilized bacteria in gel beads: a comparative study of fixation methodsJournal of Microbiological Methods, 1990
- Determination of the effective diffusion coefficient of oxygen in gel materials in relation to gel concentrationBiotechnology Techniques, 1989
- Biodegradation by immobilized bacteria in an airlift‐loop reactor—influence of biofilm diffusion limitationBiotechnology & Bioengineering, 1988
- Effectiveness factors for bioparticles with Monod kineticsThe Chemical Engineering Journal, 1988
- Immobilization of biocatalysts in thermogels using the resonance nozzle for rapid drop formation and an organic solvent for gellingBiotechnology Techniques, 1988
- Rules for optimization of biocatalysis in organic solventsBiotechnology & Bioengineering, 1987
- Comparative study of the growth of two strains ofNitrobacter in batch and continuous cultureMicrobial Ecology, 1984
- Liquid—Solid Mass Transfer in Aerated SuspensionsThe Chemical Engineering Journal, 1981
- Rapid measurement of monod half‐velocity coefficients for bacterial kineticsBiotechnology & Bioengineering, 1975
- Phosphate requirements of the nitrifying bacteriaAntonie van Leeuwenhoek, 1967